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KMID : 0380620130450010025
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Korean Journal of Food Science and Technology
2013 Volume.45 No. 1 p.25 ~ p.33
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Improvement of an Simultaneous Determination for Clenbuterol and Ractopamine in Livestock Products using LC-MS/MS
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Cho Yoon-Jae
Chae Young-Sik
Kim Hyung-Soo
Oh Jae-Ho
Kwon Ki-Sung
Chang Moon-Ik
Kim Jae-Young
Kang Il-Hyun
Do Jung-Ah
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Abstract
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Clenbuterol and ractopamine, which are β-agonists, have been misused as a growth promoting agent in meat
producing animals. Clenbuterol was banned for veterinary drug in Korea because of its problems regarding safety. Due to
their adverse effects, such as cardiovascular and central nervous diseases on human health proper control and monitoring
should be conducted. The existing analytical method of clenbuterol and ractopamine in the Food code was improved
through our present study. The bovine muscle samples were subjected to enzymatic hydrolysis, extracted with ethyl acetate
and defatted by hexane-methanol partitioning. A molecular imprinted polymer (MIP) solid phase extraction cartridge was
used for clean-up and LC-MS/MS was operated in positive multiple reaction monitoring (MRM). Clenbuterol-d9 and
ractopamine-d3 were used as an internal standard. The renewed method was validated according to the CODEX guideline.
The limits of quantitation for clenbuterol and ractopamine were 0.2 and 0.5 μg/kg, respectively. The mean recoveries
ranged in 104.2-113.5% for clenbuterol and in 107.6-118.1% for ractopamine. The improved method was able to save both
time and expenses.
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KEYWORD
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clenbuterol, ractopamine, beef, pork, LC-MS/MS
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